In vitro propagation of the endangered orchid Dendrobium chryseum Rolfefrom protocorms culture

  • Sabitri Maharjan Central Department of Botany, TU
  • Laxmi Sen Thakuri Annapurna Research Center, Kathmandu
  • Bir Bahadur Thapa Central Department of Botany, TU
  • Shreeti Pradhan Central Department of Botany, TU
  • Krishna K. Pant Central Department of Botany, TU
  • Giri P .Joshi Central Department of Botany, TU
  • Bijaya Pant Central Department of Botany, TU
Keywords: Dendrobium, culture, hormone, medium, micropropagation, rooting

Abstract

The immature seeds of Dendrobium chryseum, a sympodial epiphytic orchid with yellow flowers, were cultured in vitro, and the resultant protocorms were used as explants for seedling development. Protocorms were cultured on ½ MS medium fortified with Kinetin (Kn), 6-Benzylaminopurine (BAP), and Gibberellic Acid (GA3) in three concentrations (0.5mg/L, 1.0 mg/L and 2.0 mg/L) both alone and supplemented with 5% and 10% coconut water (CW). The highest number of shoots of D. chryseum developed on ½ - MS medium fortified with 2.0 mg/L of Kn and 10% CW and the longest shoots developed on ½ MS media fortified with 1.0 mg/L GA3, and 10% CW. The shoot derived from protocorms were placed in ½ MS medium fortified with three different rooting hormones, Indole -3- acetic acid (IAA), Indole -3-butyric acid (IBA) and α-Naphthalene acetic acid (NAA) in different concentrations alone as well as with each 1.0 mg/L hormone combined with 10% CW. The most effective of these media was ½ MS medium fortified with 1.5 mg/L IAA for rooting as well as for the production of longest roots. The present study could be useful for standardizing the protocol for mass propagation of the endangered orchid Dendrobium chryseum.

Published
2021-07-15
How to Cite
Maharjan, S., Thakuri, L., Thapa, B., Pradhan, S., Pant, K., .Joshi, G., & Pant, B. (2021). In vitro propagation of the endangered orchid Dendrobium chryseum Rolfefrom protocorms culture. Nepal Journal of Science and Technology, 20(2), 39-47. Retrieved from https://njst.nast.org.np/index.php/njst/article/view/13
Section
Articles